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KMID : 0352119890050010007
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Journal of Kyung Hee University
1989 Volume.5 No. 1 p.7 ~ p.20
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THE ULTRASTRUCTURE OF RAT LIVER CELLS IN ACUTE CARBON MONOXIDE POISONING
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Abstract
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In order to evaluate the effect of carbon monoxide exposure upon the hepatic parenchymal cells of rat, this study compare the ultrastructural changes of CO exposed group with normal control group by electron microscope.
This study has used Sprague-Dawley strain rat, weighed 200-220 gm.
The experimental group was exposed to 2,500 ppm CO in gas chamber for 15 minutes, 30 minutes, 1 hour and 4 hours, 5,000 ppm CO for 15 minutes, 30 minutes and 1 hour.
Significant data have been summarized as follow;
1) 2,500 ppm CO-exposure group: there was no significant change in this group, but the glycogen granule was diminished in 30 minutes exposure group. In 1 hour CO-exposure group, the mitochondria was appeared to be devoid of the dense matrix granules. The cisternal arrays of the rough endoplasmic reticulum were tortous and some of ribosomes, attached to the membrane, were detached. The Golgi complex were swollen and the glycogen content was markedly reduced. Furthermore, in 4 hours exposure group, Nuclear chromatin was condensed into small island. The shape of the mitochondria became irregular and the membrane of the RER have formed whorls and showed, the evidence of detachment of ribosome. The Golgi complex was appeared to be significant loss of lamellar structure and became vacuolated and distened. The :glycogen granules were markedly diminished in content.
2) 5,000 ppm CO-exposure group: In the 30 minutes CO-exposure group, the mitochondria was appeared todevoid of the dense matrix granules. some of ribosome were detached.
In the 1 hour CO-exposure, the chromatin was condensed into chromatin islands of along the nuclear membrane. Fragmentation and vacuolation of mitochondrial cristae were observed. The cisternal arrays of RER cisternae were dilated. Slightly vacuolated Golgi complex and markedly reduced glycogen were observed.
3) In view of these experimental findings, the results are very much consist with acute CO poisoning lead to the direct hepatic parenchymal degeneration and cell necrosis as well as derangement of cell organelles. Therefore, direct toxic effect of carbon monoxide are not displaceable results.
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